Tutorials

• Data preparation
  • Download sample datasets
  • Download and build reference genome
• Run Seeksoultools
  • Run tests
  • Parameter descriptions
• Output descriptions
• Algorithms Overview
  • step1: barcode/umi extraction
    • Structure design and description
    • Workflow
      • Anchor determination
      • Barcode and linker correction
    • Adaptors and polyA sequence trimming
  • step2: Alignment
    • Sequence Alignment
  • step3: Counting
    • UMI counting
    • UMI correction
    • Cell calling
  • step4: Downstream analysis
    • Seurat analysis