SeekSoulTools

SeekSoulTools is a software developed by SEEKGENE for processing single-cell transcriptome data. Currently, the software contains five modules.

  1. rna module: This module is used for identifying cell barcode, genome alignment and gene quantification to obtain a feature-barcode matrix that can be used for downstream analsis, followed by cell clustering and differential analysis. This module not only support data from SeekOne™ transcriptome kits, it also supports a variety of customized structure designs.

  2. fast module: This module is specifically designed for data produced by the SeekOne™ DD Single Cell Full-length RNA Sequence Transcriptome-seq Kit, which is used for barcode extraction, paired-end read alignment, quantification, and unique metrics analysis for full-length transcriptome data.

  3. vdj module: This module is specifically designed for data produced by the SeekOne™ DD Single Cell Immune Profiling Kit, which is used for assembling, filtering, and annotating immune receptors.

  4. multivdj module: This module is used for joint analysis of 5' RNA and VDJ data.

  5. utils module: The module contains other small tools.