Data preparation
Download sample datasets
sample datasets - md5: 878de2833feea2deb7d79224409d0d09 (Species: Homo sapiens.)
wget -c -O cellline.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/cellline.tar.gz"
# decompress
tar zxf cellline.tar.gz
curl -C - -o cellline.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/cellline.tar.gz"
# decompress
tar zxf cellline.tar.gz
Download and assembly of reference genome
Download-human-reference-GRCh38 - md5: 5473213ae62ebf35326a85c8fba6cc42
Download-hg38-rRNA - md5: 9949f6cea38633daf1d5bf1a2b976488
Download-mouse-reference-mm10 - md5: 21c246753b1f426e17d3dd00343f89be
Download-mouse-rRNA - md5: 7a1c2d573086fa9240c8978bb8a859f7
wget -c -O GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz
wget -c -O hg38_rRNA.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/hg38_rRNA.tar.gz"
tar -zxvf hg38_rRNA.tar.gz
wget -c -O mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_ensemble_102.tar.gz"
tar -zxvf mm10.tar.gz
wget -c -O mouse_rRNA.tar.gz "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_rRNA.tar.gz"
tar -zxvf mouse_rRNA.tar.gz
curl -C - -o GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz
curl -C - -o hg38_rRNA.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/hg38_rRNA.tar.gz"
tar -zxvf hg38_rRNA.tar.gz
curl -C - -o mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_ensemble_102.tar.gz"
tar -zxvf mm10.tar.gz
curl -C - -o mouse_rRNA.tar.gz "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_rRNA.tar.gz"
tar -zxvf mouse_rRNA.tar.gz
The assembly of the reference genome refers to How to build reference genome?