addtag

Modify BAM files to include barcode and UMI tags.

Run tests

Set up necessary configuration files for analysis, including the sample’s BAM file and the umi.xls file in the step3 directory. Run the SeekSoulTools using the following

seeksoultools utils addtag \
    --inbam step2/featureCounts/Samplename_SortedByName.bam \
    --umifile step3/umi.xls \
    --outbam Samplename_addtag.bam

Parameter descriptions

Parameters	Descriptions
–inbam	sample’s BAM file in the step2/featureCount directory.
–outbam	The path of the BAM file with added tags.
–umifile	he path of the umi.xls file in the step3 directory。