数据准备

测试数据下载

测试数据 - md5: 878de2833feea2deb7d79224409d0d09(物种:人)

wget -c -O cellline.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/cellline.tar.gz"
# decompress
tar zxf cellline.tar.gz
curl -C - -o cellline.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/cellline.tar.gz"
# decompress
tar zxf cellline.tar.gz

参考基因组下载和构建

Download-human-reference-GRCh38 - md5: 5473213ae62ebf35326a85c8fba6cc42

Download-hg38-rRNA - md5: 9949f6cea38633daf1d5bf1a2b976488

Download-mouse-reference-mm10 - md5: 21c246753b1f426e17d3dd00343f89be

Download-mouse-rRNA - md5: 7a1c2d573086fa9240c8978bb8a859f7

wget -c -O GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz

wget -c -O hg38_rRNA.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/hg38_rRNA.tar.gz"
tar -zxvf hg38_rRNA.tar.gz

wget -c -O mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_ensemble_102.tar.gz"
tar -zxvf mm10.tar.gz

wget -c -O mouse_rRNA.tar.gz "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_rRNA.tar.gz"
tar -zxvf mouse_rRNA.tar.gz
curl -C - -o GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz

curl -C - -o hg38_rRNA.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/hg38_rRNA.tar.gz"
tar -zxvf hg38_rRNA.tar.gz

curl -C - -o mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_ensemble_102.tar.gz"
tar -zxvf mm10.tar.gz

curl -C - -o mouse_rRNA.tar.gz "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_rRNA.tar.gz"
tar -zxvf mouse_rRNA.tar.gz

参考基因组的构建可以参考如何构建参考基因组?