Data preparation

Download sample datasets

sample datasets - md5: 3d15fcfdefc0722735d726f40ec4e324 (Species: Homo sapiens.)

wget

wget -c -O demo_dd.tar "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/demo_dd.tar"
# decompress
tar xf demo_dd.tar

curl

curl -C - -o demo_dd.tar "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/demo_dd.tar"
# decompress
tar xf demo_dd.tar

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Download and build reference genome

Download-human-reference-GRCh38 - md5: 5473213ae62ebf35326a85c8fba6cc42

Download-mouse-reference-mm10 - md5: 5c7c63701ffd7bb5e6b2b9c2b650e3c2

wget

wget -c -O GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz
wget -c -O mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10.tar.gz"

tar -zxvf mm10.tar.gz

curl

curl -C - -o GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz

curl -C - -o mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10.tar.gz"

tar -zxvf mm10.tar.gz

The assembly of the reference genome refers to How to build reference genome？