Data preparation

Download sample datasets

sample datasets - md5: 20b0c7e48cb520d10de5c4b5ee9e0486 (Species: Homo sapiens.)

wget

wget -c -O PBMC.tar "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/PBMC.tar"
# decompress
tar xf PBMC.tar

curl

curl -C - -o PBMC.tar "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/demodata/PBMC.tar"
# decompress
tar xf PBMC.tar

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Download and assembly of reference genome

Download-human-reference-GRCh38 - md5: 5473213ae62ebf35326a85c8fba6cc42

Download-hg38-rRNA - md5: 9949f6cea38633daf1d5bf1a2b976488

Download-mouse-reference-mm10 - md5: 21c246753b1f426e17d3dd00343f89be

Download-mouse-rRNA - md5: 7a1c2d573086fa9240c8978bb8a859f7

wget

wget -c -O GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz

wget -c -O hg38_rRNA.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/hg38_rRNA.tar.gz"
tar -zxvf hg38_rRNA.tar.gz

wget -c -O mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_ensemble_102.tar.gz"
tar -zxvf mm10.tar.gz

wget -c -O mouse_rRNA.tar.gz "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_rRNA.tar.gz"
tar -zxvf mouse_rRNA.tar.gz

curl

curl -C - -o GRCh38.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/GRCh38.tar.gz"
# decompress
tar -zxvf GRCh38.tar.gz

curl -C - -o hg38_rRNA.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/hg38_rRNA.tar.gz"
tar -zxvf hg38_rRNA.tar.gz

curl -C - -o mm10.tar.gz "https://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_ensemble_102.tar.gz"
tar -zxvf mm10.tar.gz

curl -C - -o mouse_rRNA.tar.gz "http://seekgene-public.oss-cn-beijing.aliyuncs.com/software/data/reference/mm10_rRNA.tar.gz"
tar -zxvf mouse_rRNA.tar.gz

The assembly of the reference genome refers to How to build reference genome?